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360 MMP FRET Substrate III
| Overview | |
|---|---|
| Description | Optimized for both 72-kDa fibroblast gelatinase(MMP 2) and 92-kDa neutrophil gelatinase(MMP 9), this peptide substrate has the fluorescent Trp residue in subsite P €™2 and the dinitrophenol (Dnp) quenching group on the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P €™1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P €™1 and P €™3 are optimized for MMP, while Arg is placed in P €™4 to enhance solubility. The water solubility of this peptide substrate in assay buffer exceeds the Km value for the enzyme reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm. |
| Sequence | Dnp-PLGMWSR |
| Sequence (3 Letter) | Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH |
| Molecular Weight | 1012.1 |
| Properties | |
| Purity | > 95% By HPLC |
| Storage | Store at -20 °C, cap vial tightly at all times. |
Ref: d’Ortho, MP. et al. Eur. J. Biochem. 250, 751 (1997); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
About InnoPep
InnoPep Inc. is a company started by a couple of researchers with decades of expertise in peptide synthesis and conjugation aimed at enabling.