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360 MMP FRET Substrate VI
| Overview | |
|---|---|
| Description | This substrate has a fluorescent Trp residue in subsite P €™2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P €™1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P €™1 and P €™3 is optimized for MMP-8, while Arg is placed in P €™4 to enhance solubility. Abs/Em = 280/360 nm. |
| Sequence | Dnp-PLAYWAR |
| Sequence (3 Letter) | Dnp-Pro-Leu-Ala-Tyr-Trp-Ala-Arg-OH |
| Molecular Weight | 1042.1 |
| Properties | |
| Purity | > 95% By HPLC |
| Storage | Store at -20 °C, cap vial tightly at all times. |
Ref: Aschi, M. et al. J. Comput. Aided. Mol. Des. 16, 213 (2002); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
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