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Product Code: 1385-005

Price: $156.00

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Description This peptide is a fluorescence resonance energy transfer (FRET) substrate for assaying ECE-1 and ECE-2, ACE1 and ACE2. neprilysin, thermolysin (TLN), MMP-2 and MMP9, thimet oligopeptidase, cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP1. Fluorescence of Mca (donor) is quenched by Dnp (quencher) in the intact peptide. Proteolytic cleavage of the FRET peptide separates the donor and the quencher, recovering the quenched fluorescence of Mca that is directly related to the enzyme activities and can be monitored at Ex/Em: 328nm/393nm.
Sequence Mca-RPPGFSAF-K(Dnp)
Sequence (3 Letter) Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Molecular Weight 1388.5
Purity > 95% By HPLC
Storage Store at -20 °C, cap vial tightly at all times.
Johnson, G.D. et al. Anal. Biochem. 286, 112 (2000); Mzhavia, N. et al. J. Biol. Chem. 278, 14704 (2003); Sigman, J.A. et al. Biochem. J. 388, 255 (2005);

About InnoPep

InnoPep Inc. is a company started by a couple of researchers with decades of expertise in peptide synthesis and conjugation aimed at enabling.

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