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InnoPep Bacterial Sortase Substrate I, FRET
DABCYL - LPETG - EDANS
DABCYL-LPETG-EDANS.. Product #: 3132-0100 Regular price: $201.00 $201.00

Bacterial Sortase Substrate I, FRET
DABCYL - LPETG - EDANS

Product Code: 3132-0100
Weight: 1.00mg

Price: $201.00

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This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.

Mazmanian, S. et al. PNAS 99, 2293 (2002); Ilangovan, U. et al. PNAS 98, 6056 (2001), Ton-That, H. et al. PNAS 96, 12424 (1999); W. Navarre and O. Schneewind Microbiol. Mol. Biol. Rev. 63, 174 (1999).
Chemistry
Purity % Peak Area By HPLC ≥ 95%
Molecular Weight 1015.2
Sequence DABCYL-LPETG-EDANS
Sequence (3 Letter) DABCYL - Leu - Pro - Glu - Thr - Gly - EDANS
Biology
Storage -20°C

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